The minions are small, yellow creatures evolved from single-celled organisms, and exist to serve the most villainous masters. However, after their incompetence results in the death of their masters, the minions resolve to isolate themselves in Antartcica.
Centuries later, the minions are driven into depression without a master, prompting one minion, Stuart, along with two others named Kevin and Bob, to journey to New York to search for a new master. In Orlando, the minions arrive at the Villain-Con, where villains from across the globe gather. They are recruited by Scarlet Overkill, along with her husband Herb. Meanwhile, the minions, after accidentally killing their new leader, a yeti, they resolve to find Stuart, Kevin and Bob.
Scarlet tasks the minion trio with stealing St. Edward's Crown from Queen Elizabeth II of the United Kingdom. The minions succeed, and Bob pulls the mythical sword Excalibur out of the Sword in the Stone. Bob is immediately crowned king while Elizabeth is removed from the throne, enraging Scarlet. Though Bob relinquishes authority to please Scarlet, she imprisons the minions in the dungeon to be tortured by Herb. When the minions prove unaffected by Herb's torture, he leaves to attend his wife's coronation,w hile the minions escape through the sewer to apologize to Scarlet.
The minions interrupt the coronation, causing Scarlet to order those attending to execute them. Kevin manages to escape and meets Elizabeth at a bar, where he learns the minions will be executed. While trying to rescue the others, Kevin enlarges himself with an experimental machine and destroys the Overkill residence. The other minions arrive in London as Kevin battles Scarlet. Scarlet fires a rocket at Kevin, which he swallows. The rocket explodes, seemingly killing the Overkills and shrinking Kevin to normal size. Elizabeth is crowned Queen of the United Kingdom, and awards the minions for their actions.
Scarlet and Herb reappear and steal Elizabeth's crown, but they are frozen by a young Gru, who steals it instead. Bob gives Scarlet his tiny crown to compensate, before the minions pursue Gru, seeing him as their new potential master.
- Pierre Coffin as Stuart, Kevin and Bob.
- Sandra Bullock as Scarlet Overkill.
- Jon Hamm as Herb Overkill.
- Katy Mixon as Tina.
- Hiroyuki Sanada as Sumo.
- Jennifer Saunders as Reine Elizabeth II.
- Michael Keaton as Walter Nelson.
- Allison Janney as Madge Nelson.
- Steve Coogan as Professor Flux.
- Steve Carell as Felonious Gru.